Role of Helicobacter pylori rfaJ genes (HP0159 and HP1416) in lipopolysaccharide synthesis

Anthony P Moran; Bethlehem Shiberu; John A Ferris; Yuriy A Knirel; Sof'ya N Senchenkova; Andreij V Perepelov; Per-Erik Jansson; Joanna B Goldberg

doi:10.1016/j.femsle.2004.10.004

Role of Helicobacter pylori rfaJ genes (HP0159 and HP1416) in lipopolysaccharide synthesis

FEMS Microbiol Lett. 2004 Dec 1;241(1):57-65. doi: 10.1016/j.femsle.2004.10.004.

Authors

Anthony P Moran¹, Bethlehem Shiberu, John A Ferris, Yuriy A Knirel, Sof'ya N Senchenkova, Andreij V Perepelov, Per-Erik Jansson, Joanna B Goldberg

Affiliation

¹ Department of Microbiology, National University of Ireland, Galway, Ireland.

PMID: 15556710
DOI: 10.1016/j.femsle.2004.10.004

Abstract

The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, isogenic mutants of H. pylori 26695 were generated. The LPS of mutant 26695::HP0159Kan did not express either Lewis epitope as detected by immunoblotting, whereas the control strain and 26695::HP1416Kan produced both epitopes. Structural analysis of the LPS of the mutants showed that HP0159 encodes an alpha(1,2/3)-glucosyltransferase whereas HP1416 encodes an alpha(1,2/4)-glucosyltransferase.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Genes, Bacterial / physiology*
Glucosyltransferases / genetics*
Helicobacter pylori / genetics*
Helicobacter pylori / metabolism
Lipopolysaccharides / biosynthesis*
Lipopolysaccharides / chemistry

Substances

Lipopolysaccharides
Glucosyltransferases

Abstract

Publication types

MeSH terms

Substances

Grants and funding