Monitoring of cAMP-imprinted polymer by fluorescence spectroscopy

Biosens Bioelectron. 2004 Dec 15;20(6):1031-9. doi: 10.1016/j.bios.2004.07.035.

Abstract

Conventional functional monomers together with fluorescent monomer, trans-4-[p-(N,N-dimethylamino)styryl]-N-vinylbenzylpyridinium chloride (vb-DMASP), were copolymerised in the presence of a target molecule, nucleotide-cAMP that acted as a molecular template. The polymer was copolymerised in thin-layer films. After removal of the template the functionalised cavities that exist in the fluorescent material are able to specifically bind the template. Subsequent adsorption of the template-cAMP causes quenching of fluorescence of the polymer. The specific photochemical processes accompanying the template adsorption are discussed further. The imprinted polymers monitored by both steady-state and time-resolved fluorescence techniques show specificity and selectivity of binding of the template on the imprinted functionalised cavities.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aniline Compounds / chemistry*
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Coated Materials, Biocompatible / analysis
  • Coated Materials, Biocompatible / chemistry*
  • Cyclic AMP / analysis*
  • Cyclic AMP / chemistry*
  • Pyridinium Compounds / chemistry*
  • Spectrometry, Fluorescence / instrumentation
  • Spectrometry, Fluorescence / methods*
  • Surface Properties

Substances

  • 4-(4-N,N-dimethylaminostyryl)-N-vinylbenzylopyridinium chloride
  • Aniline Compounds
  • Coated Materials, Biocompatible
  • Pyridinium Compounds
  • Cyclic AMP