RNA degradation induced by the herpes simplex virus vhs protein proceeds 5' to 3' in vitro

Jorge Perez-Parada; Holly A Saffran; James R Smiley

doi:10.1128/JVI.78.23.13391-13394.2004

RNA degradation induced by the herpes simplex virus vhs protein proceeds 5' to 3' in vitro

J Virol. 2004 Dec;78(23):13391-4. doi: 10.1128/JVI.78.23.13391-13394.2004.

Authors

Jorge Perez-Parada¹, Holly A Saffran, James R Smiley

Affiliation

¹ Department of Medical Microbiology & Immunology, 632 Heritage Medical Research Centre, University of Alberta, Edmonton, Alberta, Canada.

Abstract

The herpes simplex virus virion host shutoff protein (vhs) is an mRNA-specific RNase that contributes to shutoff of host protein synthesis. We show here that vhs-induced mRNA decay proceeds 5' to 3' in an in vitro assay system derived from rabbit reticulocyte lysate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
RNA, Messenger / metabolism*
RNA, Viral / metabolism*
Rabbits
Reticulocytes / metabolism
Ribonucleases
Ribosomes / metabolism
Viral Proteins / physiology*

Substances

RNA, Messenger
RNA, Viral
Viral Proteins
virion host shutoff protein, Simplexvirus
Ribonucleases