Light dependency of resistance to ionizing radiation in Euglena gracilis

Hirotaka Hayashi; Issay Narumi; Seiichi Wada; Masahiro Kikuchi; Masakazu Furuta; Kaku Uehara; Hiroshi Watanabe

doi:10.1016/j.jplph.2004.04.005

Light dependency of resistance to ionizing radiation in Euglena gracilis

J Plant Physiol. 2004 Oct;161(10):1101-6. doi: 10.1016/j.jplph.2004.04.005.

Authors

Hirotaka Hayashi¹, Issay Narumi, Seiichi Wada, Masahiro Kikuchi, Masakazu Furuta, Kaku Uehara, Hiroshi Watanabe

Affiliation

¹ Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1-1 Gakuencho, Sakai 599-8531, Japan.

PMID: 15535119
DOI: 10.1016/j.jplph.2004.04.005

Abstract

The resistance of Euglena gracilis strains Z (wild type) and SM-ZK (chloroplast-deficient mutant) to ionizing radiation was investigated. The colony forming ability of E. gracilis strain Z was higher than that of strain SM-ZK after 60Cogamma-irradiation. For both strains, the resistance of light-grown cells was higher than that of dark-grown cells, suggesting that the light conditions during culture contribute to the radiation resistance of E. gracilis. The comet assay showed that the ability of rejoining DNA double-strand breaks (dsb) was much higher in the light-grown cells. These results suggest that E. gracilis possesses a light-induced repair system to cope with DNA dsb.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA Damage / radiation effects
Dose-Response Relationship, Radiation
Euglena gracilis / genetics
Euglena gracilis / radiation effects*
Gamma Rays*
Light*
Mutation