Abstract
This study, conducted during 2001-2003, undertook the screening of patients with acute infectious respiratory-tract disease. A random selection of positive specimens was used for sequencing studies of the human metapneumovirus (hMPV) nucleoprotein gene and the phosphoprotein (P) gene. Australian and international sequences were compared, and a global classification scheme was developed. The hMPV P gene was an ideal molecular target for the detection and genotyping of hMPV. The region contained conserved sequences for reverse-transcriptase-polymerase chain-reaction primers and adequate variability to permit the accurate genotyping of the virus into 2 main lineages and 4 sublineages. Establishing viral identity is essential for the linking of genotype and disease severity.
Publication types
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Comparative Study
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Multicenter Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acute Disease
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Australia / epidemiology
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Child
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Family Practice
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Female
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Genes, Viral*
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Genotype
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Hospitals
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Humans
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Male
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Metapneumovirus / classification
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Metapneumovirus / genetics*
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Molecular Epidemiology
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Molecular Sequence Data
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Nucleoproteins / genetics
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Paramyxoviridae Infections / epidemiology*
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Phosphoproteins / genetics*
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Phylogeny
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Respiratory Tract Infections / epidemiology*
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Respiratory Tract Infections / virology
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Sequence Alignment
Substances
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Nucleoproteins
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Phosphoproteins
Associated data
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GENBANK/AF443830
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GENBANK/AF443831
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GENBANK/AF443832
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GENBANK/AF443833
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GENBANK/AF443834
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GENBANK/AY241585
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GENBANK/AY251034
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GENBANK/AY251035
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GENBANK/AY251036
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GENBANK/AY251037
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GENBANK/AY251038
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GENBANK/AY251039
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GENBANK/AY251040
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GENBANK/AY251041
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GENBANK/AY251042
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GENBANK/AY251043
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GENBANK/AY251044
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GENBANK/AY251045
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GENBANK/AY251046
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GENBANK/AY251047
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GENBANK/AY251048
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GENBANK/AY251049
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GENBANK/AY251050
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GENBANK/AY251051
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GENBANK/AY256863
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GENBANK/AY256864
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GENBANK/AY256865
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GENBANK/AY256866
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GENBANK/AY256867
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GENBANK/AY256873
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GENBANK/AY256874
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GENBANK/AY256875
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GENBANK/AY321501
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GENBANK/AY321502
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GENBANK/AY321503
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GENBANK/AY321504
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GENBANK/AY321505
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GENBANK/AY321506
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GENBANK/AY321507