Cold sensitivity of thermophilic and mesophilic RNA polymerases

J Bacteriol. 2004 Nov;186(22):7818-20. doi: 10.1128/JB.186.22.7818-7820.2004.

Abstract

RNA polymerase from mesophilic Deinococcus radiodurans displays the same cold sensitivity of promoter opening as RNA polymerase from the closely related thermophilic Thermus aquaticus. This suggests that, contrary to the accepted view, cold sensitivity of promoter opening by thermophilic RNA polymerases may not be a consequence of their thermostability.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cold Temperature*
  • DNA-Directed RNA Polymerases / metabolism*
  • Deinococcus / enzymology*
  • Enzyme Stability
  • Hot Temperature
  • Promoter Regions, Genetic
  • RNA, Bacterial / metabolism
  • Thermus / enzymology*
  • Transcription, Genetic

Substances

  • RNA, Bacterial
  • DNA-Directed RNA Polymerases