Abstract
To examine how substance P (SP) is related with dental pulp inflammation, we examined the effects of SP on expression of genes for inflammatory factors in human dental pulp cell cultures. Using reverse transcriptase-polymerase chain reaction, we found that Prevotella intermedia lipopolysaccharide (LPS) induced expression of SP and SP-receptor mRNAs, and that somatostatin inhibited the LPS-induced expression of SP mRNA. We also found that SP enhanced LPS-induced stimulation of NF-kappaB binding activity. In addition, SP induced expression of cyclooxygenase-2 and interleukin-10 receptor mRNAs. In contrast, SP inhibited expression of interferon-gamma receptor mRNA. These results suggest that SP may play a regulatory role in the immunological response of dental pulp tissue to pathogenic bacteria.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Autocrine Communication
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Blotting, Western
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Cells, Cultured
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Cyclooxygenase 2
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Dental Pulp / cytology
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Dental Pulp / immunology*
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Dental Pulp / metabolism*
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Fibroblasts / metabolism
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Gene Expression
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Humans
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Inflammation Mediators / metabolism*
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Interferon gamma Receptor
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Isoenzymes / biosynthesis
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Isoenzymes / genetics
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Lipopolysaccharides / pharmacology*
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Membrane Proteins
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NF-kappa B / metabolism
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Prevotella intermedia / chemistry
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Prostaglandin-Endoperoxide Synthases / biosynthesis
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Prostaglandin-Endoperoxide Synthases / genetics
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RNA, Messenger / analysis
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Receptors, Interferon / antagonists & inhibitors
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Receptors, Interleukin / biosynthesis
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Receptors, Interleukin-10
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Receptors, Neurokinin-1 / biosynthesis
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Receptors, Neurokinin-1 / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Substance P / biosynthesis
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Substance P / genetics
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Substance P / physiology*
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Up-Regulation
Substances
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Inflammation Mediators
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Isoenzymes
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Lipopolysaccharides
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Membrane Proteins
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NF-kappa B
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RNA, Messenger
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Receptors, Interferon
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Receptors, Interleukin
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Receptors, Interleukin-10
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Receptors, Neurokinin-1
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Substance P
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Cyclooxygenase 2
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PTGS2 protein, human
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Prostaglandin-Endoperoxide Synthases