Abstract
CD160 is an Ig-like activating NK cell receptor expressed on the majority of circulating NK cells. This population corresponds to the nonproliferating, highly cytolytic, CD56dimCD16+ subset. CD160 engagement by HLA-C molecules mediates cytotoxic function. In this study, we report that upon specific activation by the physiological ligand HLA-C, or Ab cross-linking, CD160+ peripheral blood NK cells produce IFN-gamma, TNF-alpha, and IL-6. This unique CD160-mediated cytokine production differs from the one observed after CD16 engagement whose expression is also restricted to the CD56dim cytotoxic NK cell subset. As already reported for the CD160-mediated cytotoxic effector function, CD160-mediated cytokine production by peripheral blood-NK cells is negatively controlled by the killer Ig-like receptor CD158b. Thus, the CD160 receptor represents a unique triggering surface molecule expressed by cytotoxic NK cells that participates in the inflammatory response and determines the type of subsequent specific immunity.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / metabolism
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Antigens, CD / biosynthesis
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Antigens, CD / blood
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Antigens, CD / immunology
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Antigens, CD / physiology*
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Cells, Cultured
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Cross-Linking Reagents / metabolism
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Cytokines / antagonists & inhibitors
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Cytokines / biosynthesis
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Cytokines / blood
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Cytokines / metabolism*
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Cytotoxicity Tests, Immunologic
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Cytotoxicity, Immunologic*
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GPI-Linked Proteins
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HLA-C Antigens / biosynthesis
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HLA-C Antigens / blood
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HLA-C Antigens / physiology*
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Humans
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Interferon-gamma / biosynthesis
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Interleukin-6 / biosynthesis
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K562 Cells
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Killer Cells, Natural / immunology*
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Killer Cells, Natural / metabolism*
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Ligands
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Lymphocyte Subsets / immunology*
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Lymphocyte Subsets / metabolism*
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Membrane Proteins / biosynthesis
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Membrane Proteins / blood
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Membrane Proteins / immunology
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Membrane Proteins / physiology*
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Receptors, IgG / blood
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Receptors, IgG / immunology
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Receptors, Immunologic / biosynthesis
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Receptors, Immunologic / blood
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Receptors, Immunologic / immunology
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Receptors, Immunologic / physiology*
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Receptors, KIR
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Receptors, KIR2DL3
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Receptors, Natural Killer Cell
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Tumor Necrosis Factor-alpha / biosynthesis
Substances
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Antibodies, Monoclonal
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Antigens, CD
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CD160 protein, human
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Cross-Linking Reagents
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Cytokines
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GPI-Linked Proteins
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HLA-C Antigens
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Interleukin-6
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Ligands
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Membrane Proteins
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Receptors, IgG
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Receptors, Immunologic
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Receptors, KIR
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Receptors, KIR2DL3
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Receptors, Natural Killer Cell
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Tumor Necrosis Factor-alpha
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Interferon-gamma