Abstract
A copper(II)-quenched molecular beacon was prepared by attaching fluorescein to the 3'-end and a copper(II) complex to the 5'-end of DNA. In the presence of complementary DNA, copper(II) and dye are spatially separated in the duplex and fluorescence increases up to 15-fold, with excellent discrimination of single base mismatches.
MeSH terms
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Base Pair Mismatch
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Biosensing Techniques / methods
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Cations, Divalent
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Copper / chemistry*
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DNA / analysis*
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Fluorescein / chemistry
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry*
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Kinetics
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Nucleic Acid Conformation
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Nucleic Acid Hybridization
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Oligonucleotide Probes / chemical synthesis
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Oligonucleotide Probes / chemistry*
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Spectrometry, Fluorescence
Substances
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Cations, Divalent
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Fluorescent Dyes
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Oligonucleotide Probes
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Copper
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DNA
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Fluorescein