Copper(II)-quenched oligonucleotide probes for fluorescent DNA sensing

J Am Chem Soc. 2004 Oct 27;126(42):13626-7. doi: 10.1021/ja047252a.

Abstract

A copper(II)-quenched molecular beacon was prepared by attaching fluorescein to the 3'-end and a copper(II) complex to the 5'-end of DNA. In the presence of complementary DNA, copper(II) and dye are spatially separated in the duplex and fluorescence increases up to 15-fold, with excellent discrimination of single base mismatches.

MeSH terms

  • Base Pair Mismatch
  • Biosensing Techniques / methods
  • Cations, Divalent
  • Copper / chemistry*
  • DNA / analysis*
  • Fluorescein / chemistry
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Kinetics
  • Nucleic Acid Conformation
  • Nucleic Acid Hybridization
  • Oligonucleotide Probes / chemical synthesis
  • Oligonucleotide Probes / chemistry*
  • Spectrometry, Fluorescence

Substances

  • Cations, Divalent
  • Fluorescent Dyes
  • Oligonucleotide Probes
  • Copper
  • DNA
  • Fluorescein