Rapid cloning and expression of a fungal polyketide synthase gene involved in squalestatin biosynthesis

Russell J Cox; Frank Glod; Deirdre Hurley; Colin M Lazarus; Thomas P Nicholson; Brian A M Rudd; Thomas J Simpson; Barrie Wilkinson; Ying Zhang

doi:10.1039/b411973h

Rapid cloning and expression of a fungal polyketide synthase gene involved in squalestatin biosynthesis

Chem Commun (Camb). 2004 Oct 21:(20):2260-1. doi: 10.1039/b411973h. Epub 2004 Sep 24.

Authors

Russell J Cox¹, Frank Glod, Deirdre Hurley, Colin M Lazarus, Thomas P Nicholson, Brian A M Rudd, Thomas J Simpson, Barrie Wilkinson, Ying Zhang

Affiliation

¹ School of Chemistry, University of Bristol, Cantock's Close, Bristol, UK BS8 1TS. r.j.cox@bris.ac.uk.

PMID: 15489970
DOI: 10.1039/b411973h

Abstract

PCR primers designed to selectively amplify the unique C-methyltransferase domain of fungal polyketide synthases were used to selectively clone a polyketide synthase gene involved in the biosynthesis of the squalene synthase inhibitor squalestatin S1 , heterologous expression of which led to the biosynthesis of the squalestatin side-chain.

MeSH terms

Ascomycota / enzymology*
Ascomycota / genetics*
Bridged Bicyclo Compounds, Heterocyclic / metabolism*
DNA, Complementary / biosynthesis
DNA, Complementary / genetics
Gene Amplification
Peptide Library
Polyketide Synthases / genetics*
Promoter Regions, Genetic
Tricarboxylic Acids / metabolism*

Substances

Bridged Bicyclo Compounds, Heterocyclic
DNA, Complementary
Peptide Library
Tricarboxylic Acids
squalestatin 1
Polyketide Synthases