Synthesis of the Lewis b hexasaccharide and HSA-conjugates thereof

Glycoconj J. 2004;21(5):251-6. doi: 10.1023/B:GLYC.0000045097.19353.73.

Abstract

An efficient and short route has been elaborated for the aminopropyl spacer equipped Leb hexasaccharide. For the preparation of HSA-conjugates of this oligosaccharide, the use of disuccinimidyl suberate (DSS) and disuccinimidyl glutarate (DSG) as cross-linker reagents has been evaluated. This conjugation method emerged as being faster and easier to monitor by standard MALDI-TOF spectrometry than squarate ester based conjugations of similar efficiency if DSS is used as cross-linker.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cross-Linking Reagents
  • Glycoconjugates / chemical synthesis*
  • Glycoconjugates / chemistry
  • Humans
  • Lewis Blood Group Antigens
  • Oligosaccharides / chemical synthesis*
  • Oligosaccharides / chemistry
  • Serum Albumin / chemistry*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Succinimides

Substances

  • 3-aminopropyl fucopyranosyl-1-2-galactopyranosyl-1-3-(fucopyranosyl-1-4)-2-acetamido-2-deoxyglucopyranosyl-1-3-galactopyranosyl-1-4-glucopyranoside
  • Cross-Linking Reagents
  • Glycoconjugates
  • Lewis Blood Group Antigens
  • Oligosaccharides
  • Serum Albumin
  • Succinimides
  • disuccinimidyl glutarate
  • disuccinimidyl suberate