Abstract
An efficient and short route has been elaborated for the aminopropyl spacer equipped Leb hexasaccharide. For the preparation of HSA-conjugates of this oligosaccharide, the use of disuccinimidyl suberate (DSS) and disuccinimidyl glutarate (DSG) as cross-linker reagents has been evaluated. This conjugation method emerged as being faster and easier to monitor by standard MALDI-TOF spectrometry than squarate ester based conjugations of similar efficiency if DSS is used as cross-linker.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Cross-Linking Reagents
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Glycoconjugates / chemical synthesis*
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Glycoconjugates / chemistry
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Humans
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Lewis Blood Group Antigens
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Oligosaccharides / chemical synthesis*
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Oligosaccharides / chemistry
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Serum Albumin / chemistry*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Succinimides
Substances
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3-aminopropyl fucopyranosyl-1-2-galactopyranosyl-1-3-(fucopyranosyl-1-4)-2-acetamido-2-deoxyglucopyranosyl-1-3-galactopyranosyl-1-4-glucopyranoside
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Cross-Linking Reagents
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Glycoconjugates
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Lewis Blood Group Antigens
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Oligosaccharides
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Serum Albumin
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Succinimides
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disuccinimidyl glutarate
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disuccinimidyl suberate