Measurements of airway surface liquid height and mucus transport by fluorescence microscopy, and of ion composition by X-ray microanalysis

Godfried M Roomans; Inna Kozlova; Harriet Nilsson; Viengphet Vanthanouvong; Brian Button; Robert Tarran

doi:10.1016/j.jcf.2004.05.029

Measurements of airway surface liquid height and mucus transport by fluorescence microscopy, and of ion composition by X-ray microanalysis

J Cyst Fibros. 2004 Aug:3 Suppl 2:135-9. doi: 10.1016/j.jcf.2004.05.029.

Authors

Godfried M Roomans¹, Inna Kozlova, Harriet Nilsson, Viengphet Vanthanouvong, Brian Button, Robert Tarran

Affiliation

¹ Department of Medical Cell Biology, University of Uppsala, Box 571, SE-75123 Uppsala, Sweden. godfried.roomans@medcellbiol.uu.se

PMID: 15463946
DOI: 10.1016/j.jcf.2004.05.029

Abstract

The respiratory tract is lined by a thin layer of fluid, the airway surface liquid (ASL), which plays a critical role in lung defense. The paper describes methods to determine the height of the ASL and corresponding mucus transport rates using fluorescent probes, and methods to determine the ionic composition of the ASL by X-ray microanalysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biological Transport / physiology
Body Fluids / chemistry
Body Fluids / physiology*
Cell Culture Techniques
Fluorescent Dyes
Humans
Microscopy, Confocal / methods
Microscopy, Fluorescence / methods
Models, Biological
Mucus / physiology*
Respiratory Mucosa / physiology*
Surface Tension

Substances

Fluorescent Dyes