Protein deformation of lipid hybrid bilayer membranes studied by sum frequency generation vibrational spectroscopy

Langmuir. 2004 Oct 12;20(21):8961-5. doi: 10.1021/la0484220.

Abstract

Structural deformations of lipid hybrid bilayer membranes induced by signal peptideless (SPL) proteins have been studied for the first time using the inherently surface specific nonlinear optical technique of sum frequency generation vibrational spectroscopy. Specifically, deformations of 1,2-distearoylphosphatidylglycerol(DSPG) membranes induced by interaction with FGF-1, a SPL protein which is released asa function of cellular stress through a nonclassical pathway, have been investigated. FGF-1 was found to induce lipid alkyl chain deformations in previously highly ordered DSPG membranes at the extremely low concentration of 1 nM at 60 degrees C. The deformation process was shown to exhibit a degree of reversibility upon removal of the protein by rinsing with buffer solution.

Publication types

  • Letter
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Fibroblast Growth Factor 1 / chemistry*
  • Lipid Bilayers / chemistry*
  • Membranes, Artificial*
  • Phosphatidylglycerols / chemistry*
  • Protein Conformation
  • Spectrophotometry, Infrared / methods
  • Surface Properties
  • Vibration

Substances

  • Lipid Bilayers
  • Membranes, Artificial
  • Phosphatidylglycerols
  • Fibroblast Growth Factor 1
  • distearoyl phosphatidylglycerol