The use of mice to study models of human disease has resulted in a surge of interest in developing mouse MRI. The ability to take 3D, high-resolution images of live mice allows significant insight into anatomy and function. However, with imaging times on the order of hours, high throughput of specimens has been problematic. To facilitate high throughput, concurrent imaging of multiple mice has been developed; however, this poses further complexities regarding the ease and rapidity of loading several animals. In this study, custom-built equipment was developed to streamline the preparation process and to safely maintain seven mice during a multiple-mouse imaging session. Total preparation time for seven mice was approximately 24 min. ECG and temperature were monitored throughout the scan and maintained by regulating anesthetic and heating. Proof of principle was demonstrated in a 3-h imaging session of seven mice.