Abstract
The duck beta A-globin (beta AGLB) enhancer was closely linked to the duck beta A-GLB promoter, and the construct was used to study binding of nuclear proteins to specific sites of these regulatory elements. DNaseI-footprint analysis showed that the presence of the enhancer induced binding of proteins to additional sites on the promoter. The results are consistent with the looping-out model, based on specific interactions of enhancer-bound and promoter bound-proteins.
MeSH terms
-
Animals
-
DNA / metabolism
-
DNA Probes / metabolism
-
Deoxyribonuclease I / metabolism
-
Ducks / genetics*
-
Electrophoresis
-
Enhancer Elements, Genetic / genetics*
-
Globins / genetics*
-
Molecular Sequence Data
-
Nucleic Acid Conformation
-
Promoter Regions, Genetic / genetics*
-
Transcription Factors / genetics
-
Transcription Factors / metabolism*
Substances
-
DNA Probes
-
Transcription Factors
-
Globins
-
DNA
-
Deoxyribonuclease I
Associated data
-
GENBANK/S39996
-
GENBANK/S40001
-
GENBANK/S40008
-
GENBANK/S40009
-
GENBANK/S40010
-
GENBANK/S46737
-
GENBANK/X55351
-
GENBANK/X55352
-
GENBANK/X59014
-
GENBANK/X72580