Abstract
Neonatal treatment with diethylstilbestrol (DES) leads to disruption of spermatogenesis in adult animals after apparently normal testicular development during puberty indicating aberrant androgen action in DES-exposed adult hamsters. The present study determined the effects of exogenous androgens in neonatally DES-exposed hamsters. Exogenous androgens failed to reverse the disruption of spermatogenesis in DES-exposed animals. Neonatal DES exposure caused a significant decrease in seminal vesicle weight, and abnormal histology. While exogenous androgens caused a significant increase in seminal vesicle weight in control animals, they failed to restore the seminal vesicle weight and normal histology in DES-exposed animals. Northern blot and/or RT-PCR analysis revealed that (1) AR, ERalpha and ERbeta mRNA levels were unchanged in DES-exposed animals, and (2) mRNA levels for the AR-responsive genes calreticulin, SEC-23B, and ornithine decarboxylase were significantly decreased in DES-exposed animals. Our results suggest that neonatal DES exposure impairs the action of androgens on target organs in male hamsters.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Androgen Antagonists / administration & dosage
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Androgen Antagonists / toxicity*
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Androgens / physiology
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Androgens / toxicity*
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Animals
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Animals, Newborn
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Blotting, Northern
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Cricetinae
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Diethylstilbestrol / administration & dosage
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Diethylstilbestrol / toxicity*
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Dihydrotestosterone / pharmacology
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Drug Therapy, Combination
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Estrogens, Non-Steroidal / administration & dosage
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Estrogens, Non-Steroidal / toxicity*
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Gene Expression / drug effects
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Injections, Subcutaneous
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Male
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Organ Size / drug effects
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RNA, Messenger / metabolism
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Receptors, Androgen / drug effects
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Receptors, Androgen / genetics
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Receptors, Androgen / metabolism
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Receptors, Estrogen / drug effects
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Receptors, Estrogen / genetics
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Receptors, Estrogen / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Seminal Vesicles / drug effects
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Seminal Vesicles / metabolism
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Seminal Vesicles / pathology
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Spermatogenesis / drug effects*
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Spermatogenesis / physiology
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Testis / drug effects
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Testis / metabolism
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Testis / pathology
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Testosterone / physiology
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Testosterone / toxicity*
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Testosterone Propionate / pharmacology
Substances
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Androgen Antagonists
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Androgens
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Estrogens, Non-Steroidal
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RNA, Messenger
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Receptors, Androgen
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Receptors, Estrogen
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Dihydrotestosterone
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Testosterone
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Diethylstilbestrol
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Testosterone Propionate