Abstract
Curculin from Curculigo latifolia is a unique sweet protein that exhibits both sweet-tasting and taste-modifying activities. We isolated a gene that encodes a novel protein highly homologous to curculin. Using cDNAs of the previously known curculin (designated as curculin1) and the novel curculin isoform (curculin2), we produced a panel of homodimeric and heterodimeric recombinant curculins by Escherichia coli expression systems. It was revealed that sweet-tasting and taste-modifying activities were exhibited solely by the heterodimer of curculin1 and curculin2.
Publication types
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Clinical Trial
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Circular Dichroism
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Cloning, Molecular
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Curculigo / chemistry*
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Curculigo / genetics*
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Dimerization
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Disulfides / metabolism
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Humans
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Molecular Sequence Data
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Oxidation-Reduction
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Plant Proteins / chemistry*
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Plant Proteins / genetics
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Plant Proteins / pharmacology*
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Protein Structure, Quaternary
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Protein Subunits / chemistry
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Protein Subunits / genetics
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Protein Subunits / pharmacology
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / pharmacology
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Sweetening Agents / chemistry*
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Sweetening Agents / pharmacology*
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Taste / drug effects*
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Taste / physiology
Substances
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Disulfides
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Plant Proteins
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Protein Subunits
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Recombinant Proteins
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Sweetening Agents
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curculin