Crystallization and preliminary X-ray analysis of the laccase from Coprinus cinereus

V Ducros; G J Davies; D M Lawson; K S Wilson; S H Brown; P Østergaard; A H Pedersen; P Schneider; D S Yaver; A M Brzozowski

doi:10.1107/S0907444997004897

Crystallization and preliminary X-ray analysis of the laccase from Coprinus cinereus

Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):605-7. doi: 10.1107/S0907444997004897.

Authors

V Ducros¹, G J Davies, D M Lawson, K S Wilson, S H Brown, P Østergaard, A H Pedersen, P Schneider, D S Yaver, A M Brzozowski

Affiliation

¹ Department of Chemistry, University of York, Heslington, England.

PMID: 15299893
DOI: 10.1107/S0907444997004897

Abstract

The laccase from the fungus Coprinus cinereus has been prepared and crystallized in a form suitable for X-ray diffraction analysis. Small plate-like crystals of an enzymatically deglycosylated form of the enzyme have been grown by the hanging-drop method using polyethylene glycol as precipitant. These crystals diffract to at least 2.2 A. They belong to the space group P2(1)2(1)2(1) with cell dimensions a = 45.4, b = 85.7, c = 143.1 A with a single molecule of laccase in the asymmetric unit.