Abstract
BipA is a highly conserved protein with global regulatory properties in Escherichia coli. We show here that it functions as a translation factor that is required specifically for the expression of the transcriptional modulator Fis. BipA binds to ribosomes at a site that coincides with that of elongation factor G and has a GTPase activity that is sensitive to high GDP:GTP ratios and stimulated by 70S ribosomes programmed with mRNA and aminoacylated tRNAs. The growth rate-dependent induction of BipA allows the efficient expression of Fis, thereby modulating a range of downstream processes, including DNA metabolism and type III secretion. We propose a model in which BipA destabilizes unusually strong interactions between the 5' untranslated region of fis mRNA and the ribosome. Since BipA spans phylogenetic domains, transcript-selective translational control for the 'fast-track' expression of specific mRNAs may have wider significance.
Publication types
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Research Support, Non-U.S. Gov't
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Retracted Publication
MeSH terms
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5' Untranslated Regions / genetics
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Base Sequence
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Cell Proliferation
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Enzyme Inhibitors / pharmacology
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Escherichia coli / genetics*
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Escherichia coli / growth & development
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Escherichia coli / metabolism
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Escherichia coli Proteins / antagonists & inhibitors
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Escherichia coli Proteins / biosynthesis*
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Escherichia coli Proteins / genetics*
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Escherichia coli Proteins / metabolism*
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Factor For Inversion Stimulation Protein
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GTP Phosphohydrolases / antagonists & inhibitors
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GTP Phosphohydrolases / genetics
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GTP Phosphohydrolases / metabolism*
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Gene Expression Regulation, Bacterial*
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Molecular Sequence Data
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Molecular Weight
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Nucleic Acid Conformation
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Phosphoproteins / antagonists & inhibitors
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Phosphoproteins / genetics
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Phosphoproteins / metabolism*
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Protein Binding
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Protein Biosynthesis*
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RNA, Messenger / chemistry
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RNA, Messenger / genetics
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Ribosomes / chemistry
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Ribosomes / metabolism
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Transcription Factors / biosynthesis*
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Transcription Factors / genetics*
Substances
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5' Untranslated Regions
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Enzyme Inhibitors
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Escherichia coli Proteins
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Factor For Inversion Stimulation Protein
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Fis protein, E coli
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Phosphoproteins
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RNA, Messenger
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Transcription Factors
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GTP Phosphohydrolases
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typA protein, E coli