Modified multiplex PCR method for detection of pyrogenic exotoxin genes in staphylococcal isolates

Astrid Løvseth; Semir Loncarevic; Knut G Berdal

doi:10.1128/JCM.42.8.3869-3872.2004

Modified multiplex PCR method for detection of pyrogenic exotoxin genes in staphylococcal isolates

J Clin Microbiol. 2004 Aug;42(8):3869-72. doi: 10.1128/JCM.42.8.3869-3872.2004.

Authors

Astrid Løvseth¹, Semir Loncarevic, Knut G Berdal

Affiliation

¹ National Veterinary Institute, Section of Feed and Food Microbiology, P.O. Box 8156 Dep., 0033 Oslo, Norway. astrid.lovseth@vetinst.no

Abstract

A modified multiplex PCR method for detection of nine Staphylococcus aureus enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, and sej) and one form of immunoreactive toxic shock syndrome toxin based on a previously published method (S. R. Monday and G. A. Bohach, J. Clin. Microbiol. 37:3411-3414, 1999) has been developed. The modified PCR protocol seems robust and gives reliable results.

MeSH terms

Bacterial Toxins / genetics*
Base Sequence
DNA Primers
Electrophoresis, Agar Gel
Enterotoxins / genetics*
Exotoxins / genetics*
Polymerase Chain Reaction / methods*
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Staphylococcus aureus / genetics
Staphylococcus aureus / isolation & purification*
Superantigens / genetics*

Substances

Bacterial Toxins
DNA Primers
Enterotoxins
Exotoxins
RNA, Bacterial
RNA, Ribosomal, 16S
Superantigens
enterotoxin F, Staphylococcal