The luminescence behavior of diiodofluorescein (DIF) and tetrabromofluorescein (TBF) have been investigated including the solid surface room temperature phosphorescence (SS-RTP) and the room temperature fluorescence (RTF). The luminescence intensities of the two compounds are strongest in alkaline solution. RTP lifetime of the two compounds are in the range of 130-140 ms. The RTP and RTF polarization was in the range of 0.01-0.05. The two analytical methods--SS-RTP and RTF, of the two compounds have been established.