Electrochemical detector for microchip electrophoresis of poly(dimethylsiloxane) with a three-dimensional adjustor

Ning Bao; Jing-Juan Xu; Yue-Hua Dou; Yan Cai; Hong-Yuan Chen; Xing-Hua Xia

doi:10.1016/j.chroma.2004.04.036

Electrochemical detector for microchip electrophoresis of poly(dimethylsiloxane) with a three-dimensional adjustor

J Chromatogr A. 2004 Jul 2;1041(1-2):245-8. doi: 10.1016/j.chroma.2004.04.036.

Authors

Ning Bao¹, Jing-Juan Xu, Yue-Hua Dou, Yan Cai, Hong-Yuan Chen, Xing-Hua Xia

Affiliation

¹ Department of Chemistry, The Laboratory of Life Analytical Chemistry, Nanjing University, Nanjing 210093, China.

PMID: 15281276
DOI: 10.1016/j.chroma.2004.04.036

Abstract

This paper presents an electrochemical detector for poly(dimethylsiloxane) (PDMS) microchip CE with a three-dimensional adjustor which makes it possible to accurately align a separate working electrode that can be easily fabricated in laboratory to the uncertain PDMS microchannel outlet. The substantial influence of the electrode-PDMS microchannel distance was investigated. The optimal electrode-outlet distance was found to be 10 microm for the PDMS microchannel with the width of 50 microm due to its relatively slow electroosmotic flow. Adrenaline and catechol were well separated, with a linear response range from 20 microM to 1 mM, and a detection limit of 2 microM for catechol, using carbon disk electrode (diameter of 300 microm). Furthermore, arginine and histidine can be well separated and detected directly in the PDMS microchannel using a Cu disk electrode (diameter of 150 microm).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dimethylpolysiloxanes / analysis*
Electrochemistry / instrumentation*
Electrophoresis, Capillary / instrumentation*
Electrophoresis, Capillary / methods
Silicones / analysis*

Substances

Dimethylpolysiloxanes
Silicones
baysilon