Abstract
We studied the potential role of the Duffy antigen and glycophorin A as receptors for rodent malaria parasite invasion of erythrocytes. Parasitemia increased exponentially after infection with Plasmodium berghei NK65, P. chabaudi, and P. vinckei in Duffy antigen knockout, glycophorin A knockout, and wild-type mice, indicating that the Duffy antigen and glycophorin A are not essential for these malaria parasites. However, parasitemia of the Duffy antigen knockout mice infected with P. yoelii 17XL remained constant from day 5 to 14 after infection, and then decreased, resulting in autotherapy. The treatment of P. yoelii 17XL-infected Duffy antigen knockout mice with anti-CD4 antibody increased the parasitemia 15 days after infection and the mice eventually died, indicating that CD-4-positive cells play an important role in the clearance of P. yoelii 17XL at the late stage of the infection.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Protozoan / physiology
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Antilymphocyte Serum / administration & dosage
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Blood Group Antigens
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CD4 Antigens / metabolism
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CD4-Positive T-Lymphocytes / immunology
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Duffy Blood-Group System* / genetics
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Duffy Blood-Group System* / physiology
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Erythrocytes / immunology
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Erythrocytes / parasitology
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Glycophorins / deficiency
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Glycophorins / genetics
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Glycophorins / physiology
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Malaria* / genetics
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Malaria* / immunology
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Malaria* / parasitology
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Mice
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Mice, Knockout
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Plasmodium berghei / pathogenicity
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Plasmodium chabaudi / pathogenicity
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Plasmodium yoelii* / immunology
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Plasmodium yoelii* / pathogenicity
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Plasmodium yoelii* / physiology
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Protozoan Proteins / physiology
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Receptors, Cell Surface* / deficiency
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Receptors, Cell Surface* / genetics
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Receptors, Cell Surface* / physiology
Substances
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Antigens, Protozoan
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Antilymphocyte Serum
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Blood Group Antigens
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CD4 Antigens
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Duffy antigen binding protein, Plasmodium
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Duffy Blood-Group System
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Glycophorins
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Protozoan Proteins
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Receptors, Cell Surface
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Ackr1 protein mouse