Enzyme-catalysed synthesis of galactosylated 1D- and 1L-chiro-inositol, 1D-pinitol, myo-inositol and selected derivatives using the beta-galactosidase from the thermophile Thermoanaerobacter sp. strain TP6-B1

Carbohydr Res. 2004 Aug 2;339(11):1857-71. doi: 10.1016/j.carres.2004.05.016.

Abstract

The products from the enzymatic beta-D-galactopyranosylation of 1D-chiro-inositol, 1D-pinitol, 1D-3-O-allyl-4-O-methyl-chiro-inositol, 1D-3,4-di-O-methyl-chiro-inositol, 1L-chiro-inositol and myo-inositol in combined yields ranging from 46% to 64% have been obtained using the beta-galactosidase isolated from an anaerobic extreme thermophile, Thermoanaerobacter sp. strain TP6-B1 and p-nitrophenyl beta-D-galactopyranoside as the donor. Analysis of the products from these reactions reveals information about the acceptor preferences of the enzyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Catalysis
  • Galactose / analogs & derivatives
  • Galactose / chemistry*
  • Glycosylation
  • Inositol / analogs & derivatives*
  • Inositol / chemical synthesis*
  • Methylation
  • Molecular Sequence Data
  • Stereoisomerism
  • Thermoanaerobacter / enzymology*
  • beta-Galactosidase / chemistry*
  • beta-Galactosidase / isolation & purification

Substances

  • pinitol
  • Inositol
  • beta-Galactosidase
  • Galactose