Abstract
We assessed changes of the enzyme dipeptidyl peptidase IV (DPP IV, CD26) in the context of leptin or leptin receptor deficiency. C57BL/6 mice, Leptin-deficient mice (ob/ob mice, B6.V-Lep<ob>) and Leptin-receptor-deficient mice (db/db mice, B6.Cg-m+/+Lepr) were infected with B. Calmette-Guerin (BCG) and sacrificed three days later. DPP IV activity in serum was higher in ob/ob mice and in db/db mice than in wild-type mice. The expression of DPP IV/CD26 on splenocytes was higher in ob/ob mice than in wild-type animals, and lower in db/db mice, and decreased upon stimulation with BCG in ob/ob mice only. Several T cell antigens including CTLA-4 were expressed aberrantly in ob/ob and in db/db mice. Our observations provide evidence for a relationship between DPP IV and leptin.
MeSH terms
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Animals
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Antigens, CD
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Antigens, Differentiation / genetics
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Antigens, Differentiation / immunology*
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Antigens, Differentiation / metabolism
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BCG Vaccine / immunology
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CD28 Antigens / genetics
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CD28 Antigens / immunology*
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CD28 Antigens / metabolism
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CTLA-4 Antigen
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Dipeptidyl Peptidase 4 / genetics
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Dipeptidyl Peptidase 4 / immunology*
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Dipeptidyl Peptidase 4 / metabolism
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Female
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Gene Expression Regulation, Enzymologic
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Leptin / genetics
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Leptin / immunology*
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Leptin / metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Mycobacterium bovis / immunology*
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Mycobacterium bovis / metabolism
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / immunology*
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Receptors, Cell Surface / metabolism
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Receptors, Leptin
Substances
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Antigens, CD
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Antigens, Differentiation
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BCG Vaccine
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CD28 Antigens
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CTLA-4 Antigen
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Ctla4 protein, mouse
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Leptin
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Receptors, Cell Surface
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Receptors, Leptin
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leptin receptor, mouse
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Dipeptidyl Peptidase 4