Scaling of microtubule force-velocity curves obtained at different tubulin concentrations

Phys Rev Lett. 2004 Jun 18;92(24):248101. doi: 10.1103/PhysRevLett.92.248101. Epub 2004 Jun 16.

Abstract

We present a single curve that describes the decay in average growth velocity for microtubules in response to a mechanical force. Curves obtained at two new and one previously studied tubulin concentrations coalesce when normalized with the growth velocity at zero load. This scaling provides direct evidence for a force-independent molecular off rate, in agreement with Brownian ratchet models. In addition, microtubule length changes were measured with a precision up to 10 nm, revealing that microtubules under load abruptly switch between different growth velocities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dimerization
  • Microtubules / chemistry*
  • Models, Biological
  • Molecular Motor Proteins / chemistry
  • Tubulin / chemistry*

Substances

  • Molecular Motor Proteins
  • Tubulin