Human immunodeficiency virus type 1 and hepatitis B virus transcription in peripheral blood lymphocytes from co-infected subjects

M Clementi; A Manzin; P Bagnarelli; S Menzo; P E Varaldo; G Carloni

doi:10.1007/BF01309679

Human immunodeficiency virus type 1 and hepatitis B virus transcription in peripheral blood lymphocytes from co-infected subjects

Arch Virol. 1992;126(1-4):1-9. doi: 10.1007/BF01309679.

Authors

M Clementi¹, A Manzin, P Bagnarelli, S Menzo, P E Varaldo, G Carloni

Affiliation

¹ Institute of Microbiology, University of Ancona, Italy.

PMID: 1524492
DOI: 10.1007/BF01309679

Abstract

The molecular characteristics of peripheral blood lymphocyte (PBL) infection by hepatitis B virus (HBV) were studied in human immunodeficiency virus type 1 (HIV-1) infected subjects using highly sensitive polymerase chain reaction (PCR) based techniques. DNA and RNA samples were purified from PBLs of HIV-1 infected individuals, regardless of their HBV serological status and assayed using PCR and reverse-transcription (RT) PCR, respectively. The data shown here are an extension of previous reports documenting HBV and HIV-1 co-infection of PBLs and indicate that transcriptionally active HBV infection of PBLs is detectable in a significant proportion of asymptomatic HIV-1 infected patients.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Viral / genetics
HIV Infections / blood
HIV Infections / complications
HIV Infections / microbiology*
HIV-1 / genetics*
Hepatitis B / blood
Hepatitis B / complications
Hepatitis B / microbiology*
Hepatitis B virus / genetics*
Humans
Lymphocytes / microbiology*
Polymerase Chain Reaction
RNA, Viral / genetics
Transcription, Genetic / genetics*

Substances

DNA, Viral
RNA, Viral