Abstract
A cDNA, designated as rtSH3p13, was isolated from a rat testis cDNA library. It consists of 1463 bp nuclear acids, which encodes a protein of 312 amino acids and was assigned the GenBank accession number AF227439. The deduced rtSH3p13 protein is a truncated isoform of SH3p13 as a result of mRNA alternative splicing. It is mainly expressed in the rat testis, detected in spermatids at the steps 8-19 of spermiogenesis, and found around the acrosome. During postnatal development, rtSH3p13 appears on day 18 and reaches maximum on day 60. Further experimental results suggested that rtSH3p13 forms a complex with activated epidermal growth factor receptor (EGFR) and interacts with synaptojanin I. Surprisingly, similar to SH3 domain, the V region of rtSH3p13 also inhibits endocytosis in CHO cells. Our results reveal a link between an rtSH3p13-synaptojanin-clathrin complex-mediated formation of pits and the process of spermiogenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing / genetics*
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Adaptor Proteins, Signal Transducing / metabolism*
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Alternative Splicing / genetics
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Amino Acid Sequence
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Animals
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Base Sequence
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Biological Transport / physiology*
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Brain / metabolism
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CHO Cells / metabolism
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Clathrin / chemistry
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Clathrin / physiology
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Cricetinae
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DNA, Complementary / chemistry*
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DNA, Complementary / genetics*
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DNA, Complementary / physiology
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Immunohistochemistry
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Male
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Molecular Sequence Data
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Nerve Tissue Proteins / chemistry
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Nerve Tissue Proteins / physiology
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Phosphoric Monoester Hydrolases / chemistry
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Phosphoric Monoester Hydrolases / physiology
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Proteins / metabolism*
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Rats
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Spermatogenesis / physiology*
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Testis / chemistry
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Testis / metabolism
Substances
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Adaptor Proteins, Signal Transducing
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Clathrin
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DNA, Complementary
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Nerve Tissue Proteins
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Proteins
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Sh3gl3 protein, rat
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synaptojanin
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Phosphoric Monoester Hydrolases