Characterization of rtSH3p13 gene encoding a development protein involved in vesicular traffic in spermiogenesis

Cell Res. 2004 Jun;14(3):197-207. doi: 10.1038/sj.cr.7290220.

Abstract

A cDNA, designated as rtSH3p13, was isolated from a rat testis cDNA library. It consists of 1463 bp nuclear acids, which encodes a protein of 312 amino acids and was assigned the GenBank accession number AF227439. The deduced rtSH3p13 protein is a truncated isoform of SH3p13 as a result of mRNA alternative splicing. It is mainly expressed in the rat testis, detected in spermatids at the steps 8-19 of spermiogenesis, and found around the acrosome. During postnatal development, rtSH3p13 appears on day 18 and reaches maximum on day 60. Further experimental results suggested that rtSH3p13 forms a complex with activated epidermal growth factor receptor (EGFR) and interacts with synaptojanin I. Surprisingly, similar to SH3 domain, the V region of rtSH3p13 also inhibits endocytosis in CHO cells. Our results reveal a link between an rtSH3p13-synaptojanin-clathrin complex-mediated formation of pits and the process of spermiogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics*
  • Adaptor Proteins, Signal Transducing / metabolism*
  • Alternative Splicing / genetics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Transport / physiology*
  • Brain / metabolism
  • CHO Cells / metabolism
  • Clathrin / chemistry
  • Clathrin / physiology
  • Cricetinae
  • DNA, Complementary / chemistry*
  • DNA, Complementary / genetics*
  • DNA, Complementary / physiology
  • Immunohistochemistry
  • Male
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / physiology
  • Phosphoric Monoester Hydrolases / chemistry
  • Phosphoric Monoester Hydrolases / physiology
  • Proteins / metabolism*
  • Rats
  • Spermatogenesis / physiology*
  • Testis / chemistry
  • Testis / metabolism

Substances

  • Adaptor Proteins, Signal Transducing
  • Clathrin
  • DNA, Complementary
  • Nerve Tissue Proteins
  • Proteins
  • Sh3gl3 protein, rat
  • synaptojanin
  • Phosphoric Monoester Hydrolases

Associated data

  • GENBANK/AF227439