Structure of the O-polysaccharide of the lipopolysaccharide of Azospirillum irakense KBC1

Yuliya P Fedonenko; Olga N Konnova; George V Zatonsky; Alexander S Shashkov; Svetlana A Konnova; Evelina L Zdorovenko; Vladimir V Ignatov; Yuriy A Knirel

doi:10.1016/j.carres.2004.05.013

Structure of the O-polysaccharide of the lipopolysaccharide of Azospirillum irakense KBC1

Carbohydr Res. 2004 Jul 12;339(10):1813-6. doi: 10.1016/j.carres.2004.05.013.

Authors

Yuliya P Fedonenko¹, Olga N Konnova, George V Zatonsky, Alexander S Shashkov, Svetlana A Konnova, Evelina L Zdorovenko, Vladimir V Ignatov, Yuriy A Knirel

Affiliation

¹ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049 Saratov, Russia.

PMID: 15220092
DOI: 10.1016/j.carres.2004.05.013

Abstract

The O-polysaccharide was isolated from the lipopolysaccharide of the plant-growth-promoting bacterium Azospirillum irakense KBC1 and studied by sugar and methylation analyses, Smith degradation and 1H and 13C NMR spectroscopy, including 1H, 13C HSQC and NOESY experiments for linkage and sequence analysis. The following structure of the branched hexasaccharide repeating unit of the O-polysaccharide with an unusually long side chain was established: [carbohydrate structure: see text].

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens / chemistry
Azospirillum / chemistry*
Carbohydrate Conformation
Carbohydrate Sequence
Carbohydrates / chemistry
Hydrogen
Lipopolysaccharides / chemistry*
Magnetic Resonance Spectroscopy
Methylation
Models, Chemical
Molecular Sequence Data
O Antigens / chemistry
Polysaccharides / chemistry

Substances

Antigens
Carbohydrates
Lipopolysaccharides
O Antigens
Polysaccharides
Hydrogen