Ipaf is upregulated by tumor necrosis factor-alpha in human leukemia cells

FEBS Lett. 2004 Jun 18;568(1-3):79-82. doi: 10.1016/j.febslet.2004.04.095.

Abstract

Ipaf has been associated with apoptosis, cytokine processing, and nuclear factor-kappaB activation. Here, we describe that Ipaf is highly expressed in myelomonocytic cells and that the mRNA levels of Ipaf progressively increase during differentiation of CD34(+) progenitors to granulocytes and monocytes. Additionally, treatment with tumor necrosis factor-alpha and exposure to UV radiation induced the transcriptional activation of Ipaf in human leukemia HL-60 cells. Thus, Ipaf may contribute to modulate the response of myeloid cells to genotoxic and pro-inflammatory stimuli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis Regulatory Proteins
  • Base Sequence
  • CARD Signaling Adaptor Proteins
  • Calcium-Binding Proteins / genetics
  • Calcium-Binding Proteins / physiology*
  • Cell Line, Tumor
  • DNA Primers
  • Electrophoretic Mobility Shift Assay
  • Humans
  • Leukemia / pathology
  • Leukemia / physiopathology*
  • Tumor Necrosis Factor-alpha / pharmacology*
  • Ultraviolet Rays
  • Up-Regulation / drug effects*
  • Up-Regulation / radiation effects

Substances

  • Apoptosis Regulatory Proteins
  • CARD Signaling Adaptor Proteins
  • Calcium-Binding Proteins
  • DNA Primers
  • NLRC4 protein, human
  • Tumor Necrosis Factor-alpha