High-efficiency detection of severe acute respiratory syndrome virus genetic material

J Clin Microbiol. 2004 Jun;42(6):2771-3. doi: 10.1128/JCM.42.6.2771-2773.2004.

Abstract

A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log(10) times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log(10) times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bronchoalveolar Lavage Fluid / virology
  • Humans
  • RNA, Viral / analysis*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Severe acute respiratory syndrome-related coronavirus / genetics*

Substances

  • RNA, Viral