Enhancement of Cry19Aa mosquitocidal activity against Aedes aegypti by mutations in the putative loop regions of domain II

Appl Environ Microbiol. 2004 Jun;70(6):3769-71. doi: 10.1128/AEM.70.6.3769-3771.2004.

Abstract

Improvements in the mosquitocidal activity of Bacillus thuringiensis Cry19Aa were achieved by protein engineering of putative surface loop residues in domain II through rational design. The improvement of Aedes toxicity in Cry19Aa was 42,000-fold and did not affect its toxicity against Anopheles or Culex.

Publication types

  • Evaluation Study

MeSH terms

  • Aedes / drug effects*
  • Amino Acid Sequence
  • Animals
  • Bacillus thuringiensis / metabolism
  • Bacillus thuringiensis Toxins
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / toxicity*
  • Bacterial Toxins / chemistry*
  • Bacterial Toxins / genetics
  • Bacterial Toxins / toxicity*
  • Endotoxins / chemistry*
  • Endotoxins / genetics
  • Endotoxins / toxicity*
  • Hemolysin Proteins
  • Models, Molecular
  • Molecular Sequence Data
  • Mutation*
  • Pest Control, Biological*
  • Protein Engineering / methods*

Substances

  • Bacillus thuringiensis Toxins
  • Bacterial Proteins
  • Bacterial Toxins
  • Endotoxins
  • Hemolysin Proteins
  • insecticidal crystal protein, Bacillus Thuringiensis