We report on the localization of GPXle-1, a plant glutathione peroxidase (GPX)-like protein, in the internode of Lycopersicon esculentum Mill. GPXle-1 was detected in the cytoplasm near to the plasma membrane of trichomes, in the wall of collenchyma and in both the cytoplasm and wall of vascular tissues. GPXle-1 was not found in the epidermis or parenchyma. After mechanical stimulation, a change in its cell distribution was recorded. In stimulated plants, GPXle-1 was detected throughout the cytoplasm in the epidermis, collenchyma and cortical parenchyma.