Immune responses to a GnRH-based anti-fertility immunogen, induced by different adjuvants and subsequent effect on vaccine efficacy

Vaccine. 2004 Feb 25;22(8):1024-31. doi: 10.1016/j.vaccine.2003.08.043.

Abstract

A modified GnRH peptide (CHWSYGLRPG-NH2) was conjugated to tetanus toxoid and formulated with different adjuvants (non-ionic surfactant vesicles, aluminium hydroxide, Quil A, PLGA (poly(lactide-co-glycolide)/triacetin), and Quil A/PLGA). A comparison of the anti-fertility efficacy of the formulations was made by examining specific antibody levels, antibody subclasses, endocrine ablation and gonadal atrophy. The production of IgG2b antibody provided the best correlation for castration. PLGA was considered the most effective adjuvant as it produced a consistent anti-fertility response in all the treated animals.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / pharmacology*
  • Animals
  • Antibody Formation
  • Gonadotropin-Releasing Hormone / chemistry
  • Gonadotropin-Releasing Hormone / immunology*
  • Gonadotropins / blood
  • Immunization
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Male
  • Oligopeptides / chemistry
  • Oligopeptides / immunology*
  • Organ Size / drug effects
  • Organ Size / immunology
  • Rats
  • Rats, Sprague-Dawley
  • Testis / drug effects
  • Testis / immunology
  • Testosterone / blood
  • Tetanus Toxoid / chemistry
  • Vaccines, Contraceptive / chemistry
  • Vaccines, Contraceptive / immunology*

Substances

  • Adjuvants, Immunologic
  • Gonadotropins
  • Immunoglobulin G
  • Immunoglobulin M
  • Oligopeptides
  • TT-CHWSYGLRPG-NH2
  • Tetanus Toxoid
  • Vaccines, Contraceptive
  • Gonadotropin-Releasing Hormone
  • Testosterone