FN distribution in ACC-2 and high lung-metastatic ACC-M cells was studied with immunohistochemical staining.There were less FN found in ACC-2,but much more FN were observed in the highly metastatic clone Acc-M.In vitro motility of two cell lines was measured by Boyden chamber.With the presence of exgenous FN,Acc-2 became activated with a higher migration rate.The cell adhesion test showed that Acc-2 had stronger ability of adhesion than Acc-M with the presence of FN.The result suggested FN may play a vital role in the invasion and metastasis of ACC.