Abstract
Porphyromonas gingivalis is a bacterial pathogen that produces the polyproteins RgpA and Kgp, which are proteolytically processed into proteinases and adhesins. We have demonstrated that the RgpA and Kgp proteinases and adhesins are C terminally processed by carboxypeptidase CPG70 by sequencing C-terminal peptides from both the wild type and an isogenic CPG70 mutant, using ion trap mass spectrometry.
MeSH terms
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Adhesins, Bacterial / metabolism*
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Amino Acid Sequence
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Carboxypeptidases / genetics
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Carboxypeptidases / metabolism*
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Cysteine Endopeptidases / chemistry
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Cysteine Endopeptidases / metabolism*
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Gingipain Cysteine Endopeptidases
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Hemagglutinins / chemistry
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Hemagglutinins / metabolism*
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Humans
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Mass Spectrometry / methods
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Molecular Sequence Data
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Porphyromonas gingivalis / enzymology
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Porphyromonas gingivalis / genetics
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Porphyromonas gingivalis / metabolism*
Substances
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Adhesins, Bacterial
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Gingipain Cysteine Endopeptidases
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Hemagglutinins
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Carboxypeptidases
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Cysteine Endopeptidases