DNA interactions of cobalt(III) mixed-polypyridyl complexes containing asymmetric ligands

J Inorg Biochem. 2004 Jun;98(6):1143-50. doi: 10.1016/j.jinorgbio.2004.04.003.

Abstract

Three novel asymmetric ligands, 3-(pyridine-2-yl)-5,6-diphenyl-as-triazine (pdtb), 3-(pyridine-2-yl)-as-triazino[5,6-f]acenaphthylene (pdta) and 3-(pyridine-2-yl)-as-triazino[5,6-f]phenanthroline (pdtp) and their cobalt(III) complexes have been synthesized and characterized. Binding of the three complexes with calf thymus DNA (CT-DNA) has been investigated by spectroscopic methods, viscosity, cyclic voltammetry, and electrophoresis measurements. The experimental results indicate that the size and shape of the intercalated ligand have a marked effect on the binding affinity of complexes to CT-DNA. Complexes 2 and 3 have also been found to promote cleavage of plasmid pBR322 DNA from the supercoiled form I to the open circular form II upon irradiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acenaphthenes / chemical synthesis
  • Acenaphthenes / chemistry*
  • Animals
  • Cattle
  • Cobalt / chemistry*
  • DNA / chemistry*
  • Ligands
  • Organometallic Compounds / chemical synthesis
  • Organometallic Compounds / chemistry*
  • Phenanthrolines / chemical synthesis
  • Phenanthrolines / chemistry*
  • Photochemistry
  • Plasmids / chemistry*
  • Pyridines / chemical synthesis
  • Pyridines / chemistry*
  • Triazines / chemical synthesis
  • Triazines / chemistry*

Substances

  • 3-(pyridine-2-yl)-5,6-diphenyl-triazine
  • 3-(pyridine-2-yl)-as-triazino(5,6-f)acenaphthylene
  • 3-(pyridine-2-yl)-as-triazino(5,6-f)phenanthrolin
  • Acenaphthenes
  • Ligands
  • Organometallic Compounds
  • Phenanthrolines
  • Pyridines
  • Triazines
  • Cobalt
  • DNA
  • calf thymus DNA