The effects of nitric oxide on striatal serotoninergic transmission involve multiple targets: an in vivo microdialysis study in the awake rat

Brain Res. 2004 May 22;1008(2):293-8. doi: 10.1016/j.brainres.2004.01.090.

Abstract

The role of endogenous nitric oxide (NO) in N-methyl-D-aspartate (NMDA)-induced modulation of serotonin (5-HT) release in the striatum of freely moving rats has been studied using microdialysis technique. NMDA-induced increase in 5-HT release was significantly inhibited by selective nitric oxide synthase (nNOS) inhibitor S-methylthiocitrulline (S-Me-TC), ONOO- scavenger L-cysteine (L-cys), and guanylate cyclase (GC) inhibitor 1H[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ). These data suggest that modulation of 5-HT levels is linked to the formation of NO produced by NMDA receptor activation and that endogenously produced NO increases 5-HT concentrations both by stimulating formation of 3'-5'-cyclic monophosphate (cGMP) and conversion of ONOO-.

MeSH terms

  • Animals
  • Cyclic GMP / physiology
  • Enzyme Inhibitors
  • Free Radical Scavengers / pharmacology
  • Guanylate Cyclase / physiology
  • Male
  • Microdialysis
  • N-Methylaspartate / physiology
  • Neostriatum / drug effects*
  • Neostriatum / metabolism
  • Nitric Oxide / pharmacology*
  • Nitric Oxide Synthase / antagonists & inhibitors
  • Nitric Oxide Synthase Type I
  • Peroxynitrous Acid / metabolism
  • Rats
  • Rats, Wistar
  • Serotonin / metabolism
  • Serotonin / physiology*
  • Signal Transduction / drug effects
  • Superoxides / metabolism
  • Synaptic Transmission / drug effects*

Substances

  • Enzyme Inhibitors
  • Free Radical Scavengers
  • Superoxides
  • Peroxynitrous Acid
  • Nitric Oxide
  • Serotonin
  • N-Methylaspartate
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type I
  • Nos1 protein, rat
  • Guanylate Cyclase
  • Cyclic GMP