Construction and use of PCR primers from a 65 kDa mannoprotein gene for identification of C. albicans

Mol Cell Probes. 2004 Jun;18(3):171-5. doi: 10.1016/j.mcp.2003.11.005.

Abstract

A method for detection of Candida albicans in biological samples (blood, serum, urine) was developed by the use of polymerase chain reaction (PCR) amplification of a DNA fragment of a gene coding for a 65 kDa mannoprotein of C. albicans (CaMP65). The PCR amplifies a 220 bp fragments whose specificity for C. albicans was demonstrated by Southern blot with a non-radioactive probe, leading to the differentiation from all other yeast species or human and bacterial DNA. The sensitivity of this assay was 5-10 C. albicans cells per milliliter of biological sample.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Southern
  • Candida albicans / genetics*
  • Candida albicans / isolation & purification*
  • Candidiasis / microbiology
  • DNA Primers / genetics*
  • DNA, Fungal / analysis
  • DNA, Fungal / genetics
  • Genes, Fungal / genetics
  • Humans
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / genetics*
  • Molecular Weight
  • Mycological Typing Techniques / methods*
  • Polymerase Chain Reaction / methods*
  • Species Specificity

Substances

  • DNA Primers
  • DNA, Fungal
  • Membrane Glycoproteins
  • mannoproteins