Abstract
Mouse BALB/3T3-A31-1-1 (A31) cells are non-permissive to bovine herpes virus-1 (BHV-1) but permissive to pseudorabies virus (PrV). The promoter activity of the immediate early gene of BHV-1 (BICP4) was very weak when compared with that of PrV in A31 cells. Infectious BHV-1 genomic DNA co-transfected into A31 cells with plasmids expressing BICP4 and BICP0 by a strong promoter failed to yield any progeny virus. Growth of BHV-1 in non-permissible A31 cells is restricted in many phases of the growth. The fact that expression of BICP4 and/or BICP0 in A31 cells does not improve the yield of progeny virus from infectious BHV-1 genomic DNA suggests that some more growth restrictions exist beyond the expression of BHV-1 immediate early proteins.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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BALB 3T3 Cells
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Blotting, Western
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DNA Primers
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Gene Expression Regulation, Viral*
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Herpesvirus 1, Bovine / genetics
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Herpesvirus 1, Bovine / growth & development*
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Herpesvirus 1, Suid / growth & development*
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Immediate-Early Proteins / genetics
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Mice
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Microscopy, Fluorescence
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Plasmids / genetics
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Promoter Regions, Genetic / genetics
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Trans-Activators
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Transfection
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Ubiquitin-Protein Ligases
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Viral Envelope Proteins / genetics*
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Viral Proteins
Substances
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DNA Primers
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Immediate-Early Proteins
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Trans-Activators
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Viral Envelope Proteins
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Viral Proteins
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immediate early protein BICP4, bovine herpesvirus 1
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Ubiquitin-Protein Ligases
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Vmw110 protein, Human herpesvirus 1
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bICP0 protein, Bovine herpesvirus 1