Restriction of bovine herpesvirus 1 (BHV-1) growth in non-permissive cells beyond the expression of immediate early genes

J Vet Med Sci. 2004 Apr;66(4):453-5. doi: 10.1292/jvms.66.453.

Abstract

Mouse BALB/3T3-A31-1-1 (A31) cells are non-permissive to bovine herpes virus-1 (BHV-1) but permissive to pseudorabies virus (PrV). The promoter activity of the immediate early gene of BHV-1 (BICP4) was very weak when compared with that of PrV in A31 cells. Infectious BHV-1 genomic DNA co-transfected into A31 cells with plasmids expressing BICP4 and BICP0 by a strong promoter failed to yield any progeny virus. Growth of BHV-1 in non-permissible A31 cells is restricted in many phases of the growth. The fact that expression of BICP4 and/or BICP0 in A31 cells does not improve the yield of progeny virus from infectious BHV-1 genomic DNA suggests that some more growth restrictions exist beyond the expression of BHV-1 immediate early proteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • BALB 3T3 Cells
  • Blotting, Western
  • DNA Primers
  • Gene Expression Regulation, Viral*
  • Herpesvirus 1, Bovine / genetics
  • Herpesvirus 1, Bovine / growth & development*
  • Herpesvirus 1, Suid / growth & development*
  • Immediate-Early Proteins / genetics
  • Mice
  • Microscopy, Fluorescence
  • Plasmids / genetics
  • Promoter Regions, Genetic / genetics
  • Trans-Activators
  • Transfection
  • Ubiquitin-Protein Ligases
  • Viral Envelope Proteins / genetics*
  • Viral Proteins

Substances

  • DNA Primers
  • Immediate-Early Proteins
  • Trans-Activators
  • Viral Envelope Proteins
  • Viral Proteins
  • immediate early protein BICP4, bovine herpesvirus 1
  • Ubiquitin-Protein Ligases
  • Vmw110 protein, Human herpesvirus 1
  • bICP0 protein, Bovine herpesvirus 1