Evaluation of antiviral activity against human herpesvirus 8 (HHV-8) and Epstein-Barr virus (EBV) by a quantitative real-time PCR assay

Antiviral Res. 2004 Jun;62(3):121-3. doi: 10.1016/j.antiviral.2003.12.005.

Abstract

A real-time quantitative PCR was developed to assess antiviral activity of molecules against human herpesvirus 8 (HHV-8) and the Epstein-Barr virus (EBV). The antiviral activity of the reference molecules acyclovir, ganciclovir, cidofovir, adefovir and brivudin, as assessed by this methodology, proved very similar to the activity as determined by a DNA-DNA hybridisation method.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyclovir / pharmacology
  • Antiviral Agents / blood
  • Antiviral Agents / pharmacology*
  • Cell Line
  • Cidofovir
  • Cytosine / analogs & derivatives*
  • Cytosine / pharmacology
  • Ganciclovir / pharmacology
  • Herpesvirus 4, Human / drug effects*
  • Herpesvirus 4, Human / genetics
  • Herpesvirus 4, Human / immunology
  • Herpesvirus 8, Human / drug effects*
  • Herpesvirus 8, Human / genetics
  • Herpesvirus 8, Human / immunology
  • Humans
  • Microbial Sensitivity Tests
  • Organophosphonates*
  • Organophosphorus Compounds / pharmacology
  • Polymerase Chain Reaction

Substances

  • Antiviral Agents
  • Organophosphonates
  • Organophosphorus Compounds
  • Cytosine
  • Cidofovir
  • Ganciclovir
  • Acyclovir