Enrichment of cardiolipin content throughout the purification procedure of photosystem II

N Depalo; L Catucci; A Mallardi; A Corcelli; A Agostiano

doi:10.1016/j.bioelechem.2003.09.031

Enrichment of cardiolipin content throughout the purification procedure of photosystem II

Bioelectrochemistry. 2004 Jun;63(1-2):103-6. doi: 10.1016/j.bioelechem.2003.09.031.

Authors

N Depalo¹, L Catucci, A Mallardi, A Corcelli, A Agostiano

Affiliation

¹ Dipartimento di Chimica, Università di Bari, via Orabona 4, I-70126 Bari, Italy.

PMID: 15110257
DOI: 10.1016/j.bioelechem.2003.09.031

Abstract

Photosystem II is a multisubunit membrane complex which performs the water oxidation process in the higher plants. Core dimers and monomers of photosystem II have been isolated from thylakoid membranes by sucrose density gradient centrifugation. Lipids extracted from different photosystem II-enriched fractions obtained from spinach thylakoids have been analysed by thin layer chromatography. Cardiolipin is enriched throughout the purification of photosystem II complexes; in particular dimers contained two times more cardiolipin than their monomeric counterparts.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Cardiolipins / chemistry*
Cardiolipins / isolation & purification*
Chemical Fractionation / methods
Chromatography, Thin Layer / methods*
Photosystem II Protein Complex / chemistry*
Photosystem II Protein Complex / isolation & purification*
Plant Leaves / metabolism*
Spinacia oleracea / metabolism
Thylakoids / metabolism*

Substances

Cardiolipins
Photosystem II Protein Complex