Abstract
Bacillus subtilis aprE gene codes for the extracellular protease subtilisin. Its expression is controlled by AbrB, DegU, Hpr, SinI, SinR and Spo0A transition state protein regulators. To determine in vivo the protein-protein interactions among these regulators, we used the LexA-based bacterial genetic two-hybrid system. Our results show homo-dimerization to all the analyzed proteins and hetero-dimerization between SinR-SinI and SinR-Hpr.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus subtilis / genetics*
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Bacillus subtilis / metabolism*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Gene Expression Regulation, Bacterial / physiology*
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Genes, Regulator / genetics
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Membrane Transport Proteins / genetics
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Membrane Transport Proteins / metabolism*
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Serine Endopeptidases / genetics
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Serine Endopeptidases / metabolism*
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Transcription Factors / metabolism*
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Transcriptional Activation / physiology*
Substances
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AprE protein, Bacteria
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Bacterial Proteins
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LexA protein, Bacteria
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Membrane Transport Proteins
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Transcription Factors
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Serine Endopeptidases