Chemically amplified 19F-1H nuclear Overhauser effects

I Kuprov; P J Hore

doi:10.1016/j.jmr.2004.01.011

Chemically amplified 19F-1H nuclear Overhauser effects

J Magn Reson. 2004 May;168(1):1-7. doi: 10.1016/j.jmr.2004.01.011.

Authors

I Kuprov¹, P J Hore

Affiliation

¹ Department of Chemistry, University of Oxford, Physical and Theoretical Chemistry Laboratory, South Parks Road, Oxford OX1 3QZ, UK.

PMID: 15082243
DOI: 10.1016/j.jmr.2004.01.011

Abstract

Chemically induced dynamic nuclear polarisation (CIDNP) is explored as a source of nuclear hyperpolarisation in heteronuclear Overhauser effect experiments. A photochemical reaction proceeding through a radical pair intermediate is used to enhance (19)F nuclear magnetisation in 3-fluorotyrosine by more than an order of magnitude with a corresponding increase in the amplitudes of (19)F-(1)H cross-relaxation and cross-correlation effects. The reactions employed are cyclic and leave the sample chemically unchanged. The potential for enhancing the sensitivity of heteronuclear NOEs in (19)F-labelled proteins is discussed.