DNA polymorphism assay distinguishes isolates of Leishmania donovani that cause kala-azar from those that cause post-kala-azar dermal Leishmaniasis in humans

Gannavaram Sreenivas; B V Subba Raju; Ruchi Singh; Angamuthu Selvapandiyan; Robert Duncan; Dwijen Sarkar; Hira L Nakhasi; Poonam Salotra

doi:10.1128/JCM.42.4.1739-1741.2004

DNA polymorphism assay distinguishes isolates of Leishmania donovani that cause kala-azar from those that cause post-kala-azar dermal Leishmaniasis in humans

J Clin Microbiol. 2004 Apr;42(4):1739-41. doi: 10.1128/JCM.42.4.1739-1741.2004.

Authors

Gannavaram Sreenivas¹, B V Subba Raju, Ruchi Singh, Angamuthu Selvapandiyan, Robert Duncan, Dwijen Sarkar, Hira L Nakhasi, Poonam Salotra

Affiliation

¹ Institute of Pathology (ICMR), Safdarjung Hospital Campus, New Delhi, India.

Abstract

Leishmania donovani in India causes visceral infection (kala-azar) and dermal infection (post-kala-azar dermal leishmaniasis). We report here the identification of polymorphism in a well-defined genetic locus among the Leishmania parasites causing the visceral and dermal manifestations, in a comparison of 15 post-kala-azar dermal leishmaniasis and 12 kala-azar patient isolates.

MeSH terms

Animals
DNA Probes
DNA, Kinetoplast / analysis
DNA, Kinetoplast / genetics*
Humans
Leishmania donovani / classification*
Leishmania donovani / genetics
Leishmania donovani / isolation & purification*
Leishmaniasis, Cutaneous / parasitology*
Leishmaniasis, Visceral / complications*
Leishmaniasis, Visceral / parasitology*
Polymorphism, Genetic*
RNA, Ribosomal, 28S / genetics
Skin / parasitology

Substances

DNA Probes
DNA, Kinetoplast
RNA, Ribosomal, 28S