Standardization of MspI and HaeIII restriction karyotypes in cattle

Hereditas. 2004;140(2):154-7. doi: 10.1111/j.1601-5223.2004.01801.x.

Abstract

Cattle chromosomes were digested with two restriction endonucleases, MspI and HaeIII. The banding pattern induced by each enzyme allowed the identification and pairing of all individual chromosomes and consequently the elaboration of the karyotype. This method is rapid and technically easy, and proved to be of great utility in cattle cytogenetic studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Chromosome Banding*
  • Chromosomes / enzymology*
  • Chromosomes / genetics*
  • Deoxyribonuclease HpaII* / genetics
  • Deoxyribonucleases, Type II Site-Specific* / genetics
  • Karyotyping

Substances

  • Deoxyribonuclease HpaII
  • Deoxyribonucleases, Type II Site-Specific
  • GGCC-specific type II deoxyribonucleases