Effect of 3' terminal codon pairs with different frequency of occurrence on the expression of cat gene in Escherichia coli

Curr Microbiol. 2004 Feb;48(2):97-101. doi: 10.1007/s00284-003-4120-7.

Abstract

In a previous study, we have identified four types of 3' terminal codon pairs depending on their frequency of occurrence in the Escherichia coli genome: overrepresented, moderately represented, underrepresented, and missing. In this study, the influence of eight codon pairs belonging to these four groups on the efficiency of chloramphenicol acetyltransferase ( cat) gene expression in E. coli is examined. Our results show that the missing codon pairs CCU:UAG (Pro:Stop) and CCC:UAG (Pro:Stop) had decreasing effect, whereas another missing pair CCU:AGG (Pro:Arg) had an opposite effect on the yield of CAT protein in comparison with the wild-type cat gene.

MeSH terms

  • Bacterial Proteins / analysis
  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / genetics
  • Chloramphenicol O-Acetyltransferase / biosynthesis
  • Chloramphenicol O-Acetyltransferase / genetics*
  • Codon*
  • Codon, Terminator
  • Escherichia coli / genetics*
  • Gene Expression*
  • Genes, Bacterial
  • Mutagenesis, Site-Directed
  • Mutation
  • Protein Biosynthesis
  • RNA, Bacterial / analysis
  • RNA, Messenger / analysis
  • Transcription, Genetic
  • Transformation, Bacterial

Substances

  • Bacterial Proteins
  • Codon
  • Codon, Terminator
  • RNA, Bacterial
  • RNA, Messenger
  • Chloramphenicol O-Acetyltransferase