Determination of abamectin and doramectin in sheep faeces using HPLC with fluorescence detection

Biomed Chromatogr. 2004 Mar;18(2):117-24. doi: 10.1002/bmc.306.

Abstract

An analytical procedure for the determination of abamectin and/or doramectin in sheep faeces has been developed. Avermectins were extracted from sheep faeces with acetonitrile, clean-up using solid phase extraction (SPE) and analysed by high performance liquid chromatography (HPLC) with fl uorescence detection after derivatization with N-methylimidazole.The method has a low detection limit (1.0 ng/g of moist sheep faeces), low quanti fi cation limit (2.5 ng/g of moist sheep faeces), good recovery in the range 66.4-80.8% for abamectin and 67.7-85.5% for doramectin as well as good repeatability (>85%). The method is applicable to the study of the time pro fi le of excretion in sheep faeces and also for ecotoxicological studies of both avermectins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anthelmintics / analysis*
  • Chromatography, High Pressure Liquid / methods*
  • Feces / chemistry*
  • Ivermectin / analogs & derivatives*
  • Ivermectin / analysis*
  • Sensitivity and Specificity
  • Sheep
  • Spectrometry, Fluorescence / methods*

Substances

  • Anthelmintics
  • abamectin
  • Ivermectin
  • doramectin