Abstract
The somatostatin analog SMS 201-995 inhibits human peripheral blood lymphocytes (PBL) proliferation and here we demonstrate that it induces a significant increase in T cells IL-10 release as is evidenced in double fluorescence experiments. Seizing IL-10 by monoclonal antibody, SMS does not affect lymphocyte proliferation, suggesting that this cytokine is involved in the antiproliferative effect of these analog. We previously demonstrated that SMS inhibits T cells acting on the CD28 rather than the CD3-mediated signal in exactly the same way as does IL-10. Thus SMS inhibits human PBL activation by inducing IL-10 release and the consequent inhibition of the CD28 co-stimulatory pathway providing new perspectives on developing immunosuppressive strategies.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Antibodies / pharmacology
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Antigens, Differentiation, T-Lymphocyte / drug effects
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Antigens, Differentiation, T-Lymphocyte / metabolism
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Cell Division / drug effects
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Cells, Cultured
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Drug Interactions
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Enzyme-Linked Immunosorbent Assay / methods
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Flow Cytometry / methods
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Humans
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Interleukin-10 / immunology
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Interleukin-10 / metabolism*
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Isoantigens / pharmacology
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Leukocytes, Mononuclear / drug effects*
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Leukocytes, Mononuclear / metabolism
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Lymphocytes / drug effects*
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Lymphocytes / metabolism
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Middle Aged
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Octreotide / pharmacology*
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Phytohemagglutinins / pharmacology
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Somatostatin / analogs & derivatives*
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Time Factors
Substances
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Antibodies
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Antigens, Differentiation, T-Lymphocyte
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Isoantigens
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Phytohemagglutinins
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Interleukin-10
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Somatostatin
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Octreotide