Purpose: To develop nature acellular submandibular gland scaffold materials and observe its ultrastructures.
Methods: The fresh submandibular glands of SD rats were cell-extracted by chemical detergent and observed with transmission and scanning electron microscope.
Results: Submandibular gland cells were dissolved and destroyed under transmission electron microscope. Normal submandibular gland cells were ball, but the shape of cells disappeared after cell-extraction under scanning electron microscope. Collagen fibers were arranged as net structure.
Conclusions: Submandibular gland cells disappeared and the arrangement of collagen fibres were normal by Triton X-100 cell-extraction.